Composite

Part:BBa_K3778036:Design

Designed by: Yun Tsai   Group: iGEM21_CCU_Taiwan   (2021-09-06)


T7P-RBS-6xHis-Enterokinase cleavage site-CBD-linker- Thrombin releasable D2A21 -T7T


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 761
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 761
    Illegal NheI site found at 863
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 761
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 761
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 761
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This composite part contains T7 promoter, RBS, 6xHis, Enterokinase cleavage site, CBD, linker, Thrombin releasable D2A21, and T7 terminator. The T7 promoter, T7 terminator, and RBS are basic components for protein synthesis. The 6xHis is applied for protein purification and the enterokinase site is responsible for 6xHis removal after purification. CBD mediates the interaction with collagen and Thrombin-releasable D2A21 is the major effector for bacterial clearance.



Source

References